Identification and Origin of N-Linked b-D-N- Acetylglucosamine Monosaccharide Modifications on Arabidopsis Proteins1[W][OA]
نویسندگان
چکیده
Department of Plant Biology, Microbial and Plant Genomics Institute, St. Paul, Minnesota 55108 (Y.-C.K., N.J., N.E.O.); Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, Minnesota 55455 (M.D.S., T.W.M., B.A.W.); Center for Mass Spectrometry and Proteomics, University of Minnesota, St. Paul, Minnesota 55108 (T.W.M., B.A.W.); and Department of Chemistry (N.D.U., J.S., D.F.H.) and Department of Pathology (D.F.H.), University of Virginia, Charlottesville, Virginia 22904
منابع مشابه
Identification of a sex-linked SCAR marker for Plecoglossus altivelis and its application for identifying gender in cultivated and wild populations
Ayu (Plecoglossus altivelis), one kinds of valuable cultured fish species, show almost no morphological difference between male and female until sexual maturity. Here, we report the identification of sex-linked markers for the ayu, based on Amplified Fragment Length Polymorphism (AFLP) generated from cultured fish (15 males and 15 females) by using 63 different primer combinations. Genomic frag...
متن کاملIdentification and characterization of a strict and a promiscuous N-acetylglucosamine-1-P uridylyltransferase in Arabidopsis.
UDP-GlcNAc is an essential precursor for glycoprotein and glycolipid synthesis. In the present study, a functional nucleotidyltransferase gene from Arabidopsis encoding a 58.3 kDa GlcNAc1pUT-1 (N-acetylglucosamine-1-phosphate uridylyltransferase) was identified. In the forward reaction the enzyme catalyses the formation of UDP-N-acetylglucosamine and PPi from the respective monosaccharide 1-pho...
متن کاملGanglioside and monosaccharide inhibition of nonspecific lymphocyte mitogenicity by group A streptococcal pyrogenic exotoxins.
Group A streptococcal pyrogenic exotoxins (SPE) types A, B, and C are potent nonspecific lymphocyte mitogens. The mitogenicity of these exotoxins was inhibited by gangliosides and sialic acid, whereas concanavalin A was unaffected. The capacity of both concanavalin A and SPE-A to stimulate lymphocytes was suppressed by alpha-methyl-D-mannopyranoside. Galactose reduced the activity of SPE-C. The...
متن کاملCharacterization of N-glycans from Arabidopsis. Application to a fucose-deficient mutant.
The structures of glycans N-linked to Arabidopsis proteins have been fully identified. From immuno- and affinodetections on blots, chromatography, nuclear magnetic resonance, and glycosidase sequencing data, we show that Arabidopsis proteins are N-glycosylated by high-mannose-type N-glycans from Man5GlcNAc2 to Man9GlcNAc2, and by xylose- and fucose (Fuc)-containing oligosaccharides. However, co...
متن کاملIsolation and characterization of type IV group B Streptococcus capsular polysaccharide.
An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV GBS organisms but not with antiserum to organisms of GBS serotype Ia, Ib, II,...
متن کامل